Journal: Cell reports

Article Title: Cyclin E/CDK2 and feedback from soluble histone protein regulate the S phase burst of histone biosynthesis

doi: 10.1016/j.celrep.2023.112768

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Click-iT TM EdU Cell Proliferation Kit for Imaging , Fisher Scientific , C10340.

Techniques: Recombinant, ISH Cell Assay, Imaging, Software, Hybridization

Journal: Cancer Medicine

Article Title: The GNL3L ‐ MDM2 Interaction Drives Esophageal Squamous Cell Carcinoma Progression

doi: 10.1002/cam4.71146

Figure Lengend Snippet: The downregulation of GNL3L inhibited the malignant phenotypes in TE‐1 cell lines, including proliferation, invasion, migration, and apoptosis. (a) Cell Counting Kit‐8 (CCK8) assays and (b) EDU assays were performed to analyze cell proliferation in the TE‐1 cell line with sh‐GNL3L, sh‐NC, and Control. (c) Distribution of cell phases of TE‐1 in the different groups. (d) Wound healing assay results in the TE‐1 cell line with sh‐GNL3L, sh‐NC, and Control. (e) Invasion and migration of TE‐1 cells were assessed by Transwell assays. (f) Results of apoptosis using flow cytometry for the TE‐1 cell line in different groups (** p < 0.01).

Article Snippet: Cell fixation, cell permeability, and DAPI staining were carried out according to the manufacturer's instructions using the EdU Cell Proliferation Imaging Assay Kit (Procell, P‐CA‐047, Wuhan, China).

Techniques: Migration, Cell Counting, Control, Wound Healing Assay, Flow Cytometry

Journal: Cancer Medicine

Article Title: The GNL3L ‐ MDM2 Interaction Drives Esophageal Squamous Cell Carcinoma Progression

doi: 10.1002/cam4.71146

Figure Lengend Snippet: The downregulation of GNL3L inhibited the malignant phenotypes in ECA‐109 cell lines, including proliferation, invasion, migration, and apoptosis. (a) Efficiency of sh‐GNL3L for GNL3L in ECA‐109 cells by qRT‐PCR. (b) The protein expression of GNL3L in ECA‐109 cells. (c) CCK8 assay and (d) EDU assay were performed to analyze cell proliferation in the ECA‐109 cell line with sh‐GNL3L, sh‐NC, and Control. (e) Migration and invasion of ECA‐109 cells were assessed by Transwell assays. (f) Results of apoptosis using flow cytometry for the ECA‐109 cell line in different groups (** p < 0.01).

Article Snippet: Cell fixation, cell permeability, and DAPI staining were carried out according to the manufacturer's instructions using the EdU Cell Proliferation Imaging Assay Kit (Procell, P‐CA‐047, Wuhan, China).

Techniques: Migration, Quantitative RT-PCR, Expressing, CCK-8 Assay, EdU Assay, Control, Flow Cytometry

Journal: Cancer Medicine

Article Title: The GNL3L ‐ MDM2 Interaction Drives Esophageal Squamous Cell Carcinoma Progression

doi: 10.1002/cam4.71146

Figure Lengend Snippet: GNL3L positively regulated MDM2 expression and interacted with MDM2. (a) After GNL3L knockdown, qRT‐PCR, and western blot (WB) were performed to determine MDM2 expression in TE‐1 cells. (b) Differences in the expression of p53 and p21 in TE‐1 cells after the knockdown of GNL3L. (c) Co‐IP of GNL3L and MDM2 from ESCC cell lines. The input represents the total protein extract used in the IP. The GNL3L protein expression was normalized to that of GAPDH in ESCC cells. (IP, immunoprecipitation; IgG, negative control). (d) qRT‐PCR analysis of MDM2 and GNL3L expression in TE‐1 cells in different groups. (e) Protein expression of MDM2 and GNL3L of TE‐1 in different groups was determined by WB. (f) Cell proliferation was analyzed in different groups using the CCK8 assay for TE‐1 cell lines. (g) Proliferation was analyzed using an EdU assay. (** p < 0.01, ## p < 0.01).

Article Snippet: Cell fixation, cell permeability, and DAPI staining were carried out according to the manufacturer's instructions using the EdU Cell Proliferation Imaging Assay Kit (Procell, P‐CA‐047, Wuhan, China).

Techniques: Expressing, Knockdown, Quantitative RT-PCR, Western Blot, Co-Immunoprecipitation Assay, Immunoprecipitation, Negative Control, CCK-8 Assay, EdU Assay

Journal: Cancer Medicine

Article Title: The GNL3L ‐ MDM2 Interaction Drives Esophageal Squamous Cell Carcinoma Progression

doi: 10.1002/cam4.71146

Figure Lengend Snippet: GNL3L overexpression reversed the decreased malignant behavior of ESCC cells induced by MDM2 knockdown. (a) Protein expression of MDM2 and GNL3L in TE‐1 cells in different groups was determined by western blot (WB). (b) Cell proliferation was analyzed in different groups using an EdU assay. (c) Migration and invasion of TE‐1 cells were assessed using Transwell assays. (d) In the different groups, apoptosis was measured in TE‐1 cells using flow cytometry. (e) Expression of P53 and P21 in TE‐1 cells in different groups. (** p < 0.01, ## p < 0.01).

Article Snippet: Cell fixation, cell permeability, and DAPI staining were carried out according to the manufacturer's instructions using the EdU Cell Proliferation Imaging Assay Kit (Procell, P‐CA‐047, Wuhan, China).

Techniques: Over Expression, Knockdown, Expressing, Western Blot, EdU Assay, Migration, Flow Cytometry